Microbiological Quality of Water in Dental Units of the University of Magdalena

Calidad Microbiológica del agua en unidades odontológicas de la Universidad del Magdalena

Introduction: Water in dental units must comply with national and international regulations regarding optimal microbiological quality to be used for oral health purposes.

Objectives: Evaluate the microbiological quality of water in dental clinics

Methods: Samples were collected from taps in the sterilization area and triple syringes from the dental workstations, applying the simple random method, taking into consideration international and national standards. Once collected, they were treated and evaluated by the standard membrane filtration method using Chromocult Agar medium. The average and % CFU for E. coli and total coliforms in the dental units of the clinic were calculated and compared with the regulations. current.

Results: The average Colony Forming Units (CFU) per 100 ml for E. coli and total coliforms was 2 CFU on the first floor. On the second floor, the average for E. coli was 5,727 CFU and for total coliforms it was 2,182 CFU. A total absence of 36% E. coli and 14% total coliforms was observed on the first floor and 36% E. coli and 11% total coliforms on the second floor.

Conclusions: The study of water quality in dental units complies with international and national standards. The implementation of a HACCP program and the installation of reverse osmosis systems was proposed to ensure high standards in oral health.

1. Bayani M, Raisolvaezin K, Almasi-Hashiani A, Mirhoseini SH. Bacterial biofilm prevalence in dental unit waterlines: a systematic review and meta-analysis. BMC Oral Health. 18 de marzo de 2023;23(1):158. doi: 10.1186/s12903-023-02885-4

2. O’Donnell MJ, Boyle MA, Russell RJ, Coleman DC. Management of dental unit waterline biofilms in the 21st century. Future Microbiol. octubre de 2011;6(10):1209-26. 10.1016/j.jdent.2009.06.001
3. Mills SE. THE DENTAL UNIT WATERLINE CONTROVERSY: DEFUSING THE MYTHS, DEFINING THE SOLUTIONS. J Am Dent Assoc. 1 de octubre de 2000;131(10):1427-41. doi: doi: https://doi.org/10.2217/fmb.11.104
4. Depaola LG, Mangan D, Mills SE, Costerton W, Barbeau J, Shearer B, et al. A review of the science regarding dental unit waterlines. J Am Dent Assoc. 1 de septiembre de 2002;133(9):1199-206. doi: 10.14219/jada.archive.2002.0361
5. Kohn WG, Harte JA, Malvitz DM, Collins AS, Cleveland JL, Eklund KJ. COVER STORY Guidelines for infection control in dental health care settings—2003. J Am Dent Assoc. enero de 2004;135(1):33-47. doi: 10.14219/jada.archive.2004.0019
6. Okubo K, Ito T, Okamoto K, Yamamoto I, Mizutani H, Kawata Y, et al. Evaluation of the simulator with automatic irrigation control system designed for countermeasures of internal contamination in dental unit water lines. Heliyon. junio de 2020;6(6):e04132. doi: 10.1016/j.heliyon.2020.e04132
7. Meiller TF, Depaola LG, Kelley JI, Baqui AAMA, Turng BF, Falkler WA. DENTAL UNIT WATERLINES: BIOFILMS, DISINFECTION AND RECURRENCE. J Am Dent Assoc. 1 de enero de 1999;130(1):65-72. doi: 10.14219/jada.archive.1999.0030
8. Health C for D and R. Dental Unit Waterlines. FDA [Internet]. 15 de agosto de 2023 [citado 15 de septiembre de 2023]; Disponible en: https://www.fda.gov/medical-devices/dental-devices/dental-unit-waterlines
9. Mills SE, Porteous N, Zawada J. DENTAL UNIT WATER QUALITY: ORGANIZATION FOR SAFETY, ASEPSIS AND PREVENTION WHITE PAPER AND RECOMMENDATIONS–2018. 1(1). [Internet]. 15 de agosto de 2023 [citado 15 de septiembre de 2023]; Disponible en: https://www.osap.org/assets/docs/resources/toolkits-topics/dental-unit-water-quality-organization-for-safety-asepsis-and-prevention-white-paper-and-recommendations-2018.pdf
10. Siegel SC, Fraunhofer JAV. The effect of handpiece spray patterns on cutting efficiency. J Am Dent Assoc. 1 de febrero de 2002;133(2):184-8. doi: 10.14219/jada.archive.2002.0142
11. Barbeau J, Buhler T. Biofilms augment the number of free-living amoebae in dental unit waterlines. Res Microbiol. 1 de octubre de 2001;152(8):753-60. doi: 10.1016/s0923-2508(01)01256-6
12. ADA statement on dental unit waterlines. Adopted by the ADA Board of Trustees, December 13, 1995 and the ADA Council on Scientific Affairs, September 28, 1995. Northwest Dent. 1996;75(2):25-6. [citado 15 de septiembre de 2023]; Disponible en: https://www.ada.org/resources/research/science-and-research-institute/oral-health-topics/dental-unit-waterlines
13. Sands P, Galizzi P, editores. Council Directive 98/83/EC of 3 November 1998 on the quality of water intended for human consumption ( OJ L 330 05.12.1998 p. 32 ). En: Documents in European Community Environmental Law [Internet]. 2.a ed. Cambridge University Press; 2006 [citado 25 de septiembre de 2023]. p. 865-78. Disponible en: https://www.cambridge.org/core/product/identifier/CBO9780511610851A069/type/book_part
14. Ávila De Navia SL, Estupiñán Torres SM, Estupiñán Torres DM. Indicadores de calidad bacteriológica del agua en unidades odontológicas. Rev Fac Med. 29 de mayo de 2014;62(1):111-7. [citado 15 de septiembre de 2023]; Disponible en: http://www.scielo.org.co/pdf/rfmun/v62n1/v62n1a14.pdf
15. Costa D, Mercier A, Gravouil K, Lesobre J, Delafont V, Bousseau A, et al. Pyrosequencing analysis of bacterial diversity in dental unit waterlines. Water Res. septiembre de 2015;81:223-31. doi: 10.1016/j.watres.2015.05.065
16. Zhang Y, Ping Y, Zhou R, Wang J, Zhang G. High throughput sequencing-based analysis of microbial diversity in dental unit waterlines supports the importance of providing safe water for clínical use. J Infect Public Health. 1 de mayo de 2018;11(3):357-63. doi: 10.1016/j.jiph.2017.09.017
17. Pankhurst CL, Johnson NW, Woods RG. Microbial contamination of dental unit waterlines: the scientific argument. Int Dent J. agosto de 1998;48(4):359-68. doi: 10.1111/j.1875-595x.1998.tb00697.x
18. Arvand M, Hack A. Microbial contamination of dental unit waterlines in dental practices in Hesse, Germany: A cross-sectional study. Eur J Microbiol Immunol. marzo de 2013;3(1):49-52. doi: 10.1556/EuJMI.3.2013.1.7
19. Wirthlin MR, Marshall GW, Rowland RW. Formation and decontamination of biofilms in dental unit waterlines. J Periodontol. noviembre de 2003;74(11):1595-609. doi: 10.1902/jop.2003.74.11.1595
20. Tuvo B, Totaro M, Cristina ML, Spagnolo AM, Di Cave D, Profeti S, et al. Prevention and Control of Legionella and Pseudomonas spp. Colonization in Dental Units. Pathogens. abril de 2020;9(4):305. doi: 10.3390/pathogens9040305
21. Mazari W, Boucherit-Otmani Z, El Haci IA, Ilahi A, Boucherit K. Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques. Int Dent J. diciembre de 2018;68(6):386-92. doi: 10.1111/idj.12401
22. Spagnolo AM, Sartini M, Cristina ML. Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review. Pathog Basel Switz. 13 de agosto de 2020;9(8):651. doi: 10.3390/patógenos9080651
23. Vanessa B, Virginie M, Nathalie Q, Marie-Hélène R, Christine I. Hartmannella vermiformis can promote proliferation of Candida spp. in tap-water. Water Res. 1 de noviembre de 2012;46(17):5707-14. doi: 10.1016/j.waters.2012.07.054
24. Artini M, Scoarughi GL, Papa R, Dolci G, De Luca M, Orsini G, et al. Specific Anti Cross-Infection Measures may Help to Prevent Viral Contamination of Dental Unit Waterlines: a Pilot Study. Infection. 1 de octubre de 2008;36(5):467-71. doi: 10.1007/s15010-008-7246-5
25. Hoogenkamp MA, Brandt BW, de Soet JJ, Crielaard W. An in-vitro dynamic flow model for translational research into dental unit water system biofilms. J Microbiol Methods. abril de 2020;171:105879. doi: 10.1016/j.mimet.2020.105879
26. Campuzano S, Jiménez L, Hernández DM. La formación de biopelículas y la calidad del agua en la consulta odontológica. Nova. 10 de septiembre de 2018;16(29):39-49. [citado 15 de septiembre de 2023]; Disponible en: http://www.scielo.org.co/pdf/nova/v16n29/1794-2470-nova-16-29-00039.pdf
27. Dyck A, Exner M, Kramer A. Experimental based experiences with the introduction of a water safety plan for a multi-located university clinic and its efficacy according to WHO recommendations. BMC Public Health. 13 de marzo de 2007;7(1):34. doi: 10.1186/1471-2458-7-34
28. Garg SK, Mittal S, Kaur P. Dental unit waterline management: historical perspectives and current trends. J Investig Clin Dent. noviembre de 2012;3(4):247-52. doi: 10.1111/j.2041-1626.2012.00135.x
29. Aguirre Forero SE, Carrillo Cantillo MM, Romero I, Piraneque Gambasica NV. Estado del recurso hídrico en el campus de la Universidad del Magdalena, Colombia. Intropica. 30 de junio de 2022;72-87. [citado 15 de septiembre de 2023]; Disponible en: https://revistas.unimagdalena.edu.co/index.php/intropica/article/view/4557
30. Zambrano Gari CC, Luna Fontalvo JA. Diversidad microbiana presente en el ambiente de la clínica odontológica de la universidad del Magdalena. Intropica St Marta. 2013;8:61-8. [citado 15 de septiembre de 2023]; Disponible en: https://revistas.unimagdalena.edu.co/index.php/intropica/article/view/733/678.