Colonization by Staphylococcus aureus in an HIV-positive patient population of the city of Medellín: antimicrobial susceptibility patterns and characterization of resistance to methicillin

Colonización por Staphylococcus aureus en una población de pacientes VIH positivos de la ciudad de Medellín: perfil de sensibilidad antimicrobiana y caracterización de la resistencia a la meticilina

Published 2010-12-31

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Vol. 8 No. 14 (2010)
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Artículo Original
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Velásquez et al., L. A. (2010). Colonization by Staphylococcus aureus in an HIV-positive patient population of the city of Medellín: antimicrobial susceptibility patterns and characterization of resistance to methicillin. NOVA, 8(14). https://doi.org/10.22490/24629448.445
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https://doi.org/10.22490/24629448.445
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Luz Astrid Velásquez et al.
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    To determine the prevalence of nasal colonization of S Staphylococcus aureus, its antimicrobial sensitivity profile, methicillin-resistance in strains isolated and the relation between carrier state, immunological status and use of antimicrobials in a group of patients infected with human immunodeficiency virus (HIV) in Medellin 151 nasal samples were obtained from ambulatory patients with HIV. Antimicrobial sensitivity profile of S. aureus isolates were evaluated using the Kirby-Bauer method. In addition, we also determined minimum inhibitory concentrations (MIC) for oxacillin and vancomycin. Presence of the mecA gene in methicillin resistant S. aureus isolates (MRSA) was confirmed through polymerase chain reaction. Results: The prevalence of S. aureus nasal colonization was 37.74%. The colonization for MRSA was 1.98%, while S. aureus meticiline sensitive isolates were detected in 35.76% of the patients.   In the MRSA isolates we observed the following antibiotic sensitivity profile: erythromycin 66.6%, clindamycin 100%, gentamycin 100%, tetracyclin 66.6%, and vancomycin 100%. We confirmed the presence of mecA gene in all MRSA isaolates.   We determined the prevalence of nasal colonization for S. aureus in the studied population. No statistically significant relation was observed among the state of the carrier, the immunological status, antimicrobial therapy, hospitalization period or gender in the studied population. We correlated the microbiological findings with the presence of the mecA gene. Neither vancomycin resistance nor diminished susceptibility to glycopeptides isolates to were detected.

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    1. Velázquez Meza ME. Surgimiento y diseminación de Staphylococcus aureus Meticilinorresistente. Salud Publica Mex 2005; 47: 381-387.
    2. Daza Pérez R.M. Resistencia bacteriana a antimicrobianos: su importancia en la toma de decisiones en la práctica diaria. Inf Ter Sist Nac alud 1998; 22: 57-67.
    3. Justos JA, Handan A, Gutierrez M. Staphylococcus aureus: La Reemergencia de un Patógeno en la comunidad. 2006 Rev Biomed
    4. (4): 287-305.
    5. Echevarria Zarate J, Iglesias Quilca D. Estafilococo Meticilinorresistente, un problema actual en la emergencia de resistencia entre los
    6. Gram positivos. Rev Med Hered 2003; 14 (4): 195-199 Rev
    7. Koneman EW. Capitulo11 parte I: Estafilococos y microorganismos relacionados. En: Diagnostico Microbiológico Texto y Atlas Color. Montevideo, Uruguay: Editorial médica panamericana, 2004: 528-540
    8. Tenover FC, Lancaster MV, Hill B, Steward CD. Characterization of Staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol. 1998; 36: 1020-7.
    9. Po-Liang Lu, Lien-Chun Chin, Chien-Fang Peng, Yi-Hsiung Chiang, Tyen-Po Chen, Ling Ma, L. K Siu. Risk factors and molecular analysis of community methicillin-resistant staphylococcus aureus carriage. Journal of clinical microbiology 2005; 43 (1):132–139.
    10. Cortes JA, Gómez CA, Cuervo SI, Leal AL, GREBO. Communityacquired methicillin-resistant Staphylococcus aureus in Bogotá, Colombia: Public Health implications. Revista de salud pública, 2007; 9 (3): 448-454.
    11. Carleton H. A Ten Year Survey of Staphylococcus aureus (Saur) Isolates Causing Infections Among Gay Men and People with HIV
    12. in San Francisco: ICAAC San Francisco Septiembre 2006. pg. 123 Abstract C2-1142
    13. Wiiliamson JC. Community-Acquired MRSA (CA-MRSA) Infection Among HIV-Positive Patients Is Associated with Active Viral Replication: ICAAC San Francisco Septiembre 2006. pg. 364 Abstract K-1674
    14. A. Uche. Outcomes of MRSA Bacteremia in HIV Infected Patients: ICAAC San Francisco Septiembre 2006. pg. 324 Abstract K-779
    15. Safdar N, Narans L, Gordon B, Maki DG. Comparison of Culture Screening Methods for Detection Of Nasal Carriage of Methicillin-
    16. Resistant Staphylococcus aureus: a Prospective Study Comparing 32 Methods. J Clin Microb. 2003 41 (7): 3163-3166.
    17. Jaffe RI, Lane JD, Albury SV, Niemeyer DM. rapid Extraction from and Direct Identification in Clinical samples of Methicillin-resistant Staphylococcus aureus Using the PCR. J Clin Microb. 2000 38 (9): 2407-3412
    18. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. CLSI document M 100-S17
    19. J.S Villacian, T.Barkham, A.Earnest, N.I. Paton. Prevalence of and Risk Factors for Nasal Colonization with Sthaphylococcus aureus Among Human Immunodeficiency Virus- Positive Outpatients in Singapore. Infect Control Hosp Epidemiol 2004;25:438-440.
    20. M. Amir, J. Paul, B. Batchelor, S. Kariuki, J. Ojoo, R Waiyaki and C. Gilks. Nasopharyngeal Carriage of Staphylococcus aureus and Carriage of Tetracycline-Resistant Strains Associated with HIV-Seropositivity. Eur. J. Clin. Microbiol. Infect. Dis., 1995, 14:34-40.
    21. L Clifford McDonald, Tsai-Ling Lauderdale, Hsiu-Jung Lo, Jih-Jin Tsai and Chien-Ching Hung. Colonization of HIV-infected outpatients in Taiwan with methicillin-resistant and methicillin-susceptible Staphylococcus aureus. Int J STD AIDS.2003; 14: 473-477
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    23. DOI: http://dx.doi.org/10.22490/24629448.445
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