Dosis respuesta de diferentes inhibidores para estudios metabólicos en astrocitos con L-lactato en concentraciones perinatales.

Dosis respuesta de diferentes inhibidores para estudios metabólicos en astrocitos con L-lactato en concentraciones perinatales.

Los sistemas de transporte como las lanzaderas mitocondrial/citosólicas son importantes para las funciones integrativas del cerebro en desarrollo. Para facilitar su estudio y conocer el papel que estos sistemas de transporte puedan tener en células cerebrales se ha empleado trasadores radiactivos en cultivos primarios. En este trabajo se evaluaron las velocidades de oxidación (respiración) y lipogénesis utilizando lactato que es el principal sustrato cerebral durante la prelactancia en astrocitos en cultivo primario. Se determinó las concentraciones mínimas de inhibidores que interactúan con las principales lanzaderas de manera que no fueran tóxicos para las células, pero que su vez permitieran observar efectos metabólicos. Se utilizó L-[U-14C]- lactato (1 MCi), L-lactato (10.5 mM) en ausencia y presencia de diferentes concentraciones de inhibidores enzimáticos como el dicloroacetato (DCA), un inhibidor de la piruvato deshidrogenasa quinasa y el aminooxiacetato (AOA), un inhibidor de la aspartato aminotransferasa e inhibidores del transporte mitocondrial como el butilmalonato (BM), un inhibidor del transporte de dicarboxilatos y específicamente del L-malato y el 1,2,3-benceno tricarboxilato (BT) un inhibidor del transporte de tricarboxilatos. Los resultados sugieren que a concentraciones de 1 mM de DCA y de 5 mM de AOA, BM y BT son suficientes para evaluar efectos metabólicos en células cerebrales durante el periodo perinatal.

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82. DOI: http://dx.doi.org/10.22490/24629448.19