Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Las metodologías para el mantenimiento de cepas de referencia de Leptospira spp., requieren técnicas de conservación capaces de garantizar la viabilidad, evitar la contaminación y mantener las características genéticas originales de las cepas. La diversidad en los procesos de reorganización genética de Leptospira spp., hacen necesario el desarrollo de metodologías moleculares que permitan el seguimiento de la estabilidad genética de las cepas, bajo los diferentes métodos de conservación. Este estudio determinó el grado de conservación de los genes 16S rRNA y ompL1 de 10 serovares de referencia de la colección de Leptospira spp., pertenecientes al Sistema de Bancos de Germoplasma de la nación para la Alimentación y la Agricultura (SBGNAA); lo que permitió corroborar que estos no han sufrido cambios considerables, puesto que se evidenciaron similitudes superiores al 99,69% para 16S rRNA y de 99,02% para ompL1, en los alineamientos pareados, lo que comprueba la estabilidad genética y la pureza de las cepas de referencia de Leptospira spp., mantenidas en criopreservación en nitrógeno líquido a -196 °C y a temperatura ambiente durante ocho años aproximadamente

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